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EFFECT OF METAL IONS ON ARGINASE FROM THE KIDNEY OF LOCAL GOAT (Capra aegagrus hircus)
ABSTRACT
This research is aimed at isolating, partially purifying and determination of arginase from the kidney of a local goat. Methods used in the isolation and partial purification of the enzyme include; Ammonium sulphate precipitation, dialysis.etc. Result from this work showed that arginase isolated from a local goat has a specific activity of 0.358 with a yield of 12.12% incubation of the enzyme with different metal ions shows that the enzyme is inhibited by Nacl and Bacl2.
TABLE OF CONTENTS
CHAPTER ONE
1.0 Introduction and Literature review
1.1 Introduction
1.2 Literature review
1.2.1 History of arginase
1.3 Occurrence and distribution of arginase
1.3.1 Bacteria
1.3.2 Fungi
1.3.3 Lichens
1.3.4 Yeast
1.3.5 Insects
1.4 The substrate: Arginase
1.5 Scientific classification of local goat
1.6 Aim and Objectives
CHAPTER TWO
2.0 Materials and methods
2.1 Materials
2.1.1 Apparatus used
2.2 Methods
2.2.1 Assay
2.3.2 Protein determination
2.3.3 Purification procedure
CHAPTER THREE
3.0 Results
3.1 Purification and Homogeneity
3.2 Effect of metal ion on arginase from kidney of a local goat
CHAPTER FOUR
5.0 Discussion, Conclusion and Recommendation
5.1 Discussion
5.2 Conclusion
5.3 Recommendations
References.
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